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coomassie brilliant blue staining solution  (Bio-Rad)


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    Structured Review

    Bio-Rad coomassie brilliant blue staining solution
    Coomassie Brilliant Blue Staining Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/coomassie+brilliant+blue+staining+solution/Coomassie+Brilliant+Blue+R-250+Staining+Solution/pm42122151-87-19-32
    Average 99 stars, based on 2202 article reviews
    coomassie brilliant blue staining solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Epigenetic gene regulation is controlled by distinct regulatory complexes utilizing specialized paralogs of TELOMERE REPEAT BINDING FACTORS
    Article Snippet: Protein quantity was determined by Bio-Rad Protein Assay according to manufacturer’s instructions. .. To check protein integrity, 1 μg of protein was mixed 2x SDS-Loading buffer (126 mM Tris-HCl (pH 6.8), 20 % glycerol, 4 % SDS, 0.02 % bromophenol blue), incubated at 95 °C for 5 min, and run in 1x TGS buffer (Bio Rad) on a 1.5 mm, 12 % SDS-PAGE at 100 V for approximately 1.5 h. The SDS-PAGE was stained with Coomassie brilliant blue staining solution (1 g Coomassie Brilliant Blue (Bio-Rad), 500 ml MeOH, 100 ml glacial acetic acid, 400 ml H 2 O) and de-stained with H 2 O overnight. .. Forward and reverse 5’-Cy3-labelled oligonucleotides of 28 bp were ordered from SIGMA-ALDRICH.

    Article Title: Correction to “Incorporation of Multiple β 2 ‑Hydroxy Acids into a Protein in Vivo Using an Orthogonal Aminoacyl-tRNA Synthetase”
    Article Snippet: .. 30 μL of sample was loaded per well to a Bio-Rad Any kDTM Mini-PROTEAN® TGXTM Precast Protein Gel and run at 200 V for 30 min. Gels were incubated in coomassie brilliant blue staining solution for 30 min followed by destaining solution for 30 min and imaged on a Bio-Rad ChemiDocTM MP Imaging System. ..

    SDS Page:

    Article Title: Epigenetic gene regulation is controlled by distinct regulatory complexes utilizing specialized paralogs of TELOMERE REPEAT BINDING FACTORS
    Article Snippet: Protein quantity was determined by Bio-Rad Protein Assay according to manufacturer’s instructions. .. To check protein integrity, 1 μg of protein was mixed 2x SDS-Loading buffer (126 mM Tris-HCl (pH 6.8), 20 % glycerol, 4 % SDS, 0.02 % bromophenol blue), incubated at 95 °C for 5 min, and run in 1x TGS buffer (Bio Rad) on a 1.5 mm, 12 % SDS-PAGE at 100 V for approximately 1.5 h. The SDS-PAGE was stained with Coomassie brilliant blue staining solution (1 g Coomassie Brilliant Blue (Bio-Rad), 500 ml MeOH, 100 ml glacial acetic acid, 400 ml H 2 O) and de-stained with H 2 O overnight. .. Forward and reverse 5’-Cy3-labelled oligonucleotides of 28 bp were ordered from SIGMA-ALDRICH.

    Article Title: Loss of SALL1 Promotes Hepatocellular Carcinoma Growth and Is Associated with Poor Clinical Outcome.
    Article Snippet: Band intensities were quantified using Image Lab software (Bio-Rad Laboratories, Hercules, CA, USA) and normalized to β-actin (ACTB) as an internal control. .. For total protein visualization, whole gels loaded with 30 μg of protein per lane were stained after SDS-PAGE with Coomassie Brilliant Blue staining solution (0.1% w/v, methanol: 45% v/v, acetic acid: 10%) (Bio-Rad Laboratories, Hercules, CA, USA; Cat. No. 161-0406). .. Gels were destained for https://doi.org/10.3390/cancers18091355 several hours in solution containing 45% methanol and 10% acetic acid (Sigma-Aldrich, St. Louis, MO, USA; Cat. No. 64-19-7), and protein levels in all lanes were assessed visually.

    Article Title: Epigenetic gene regulation is controlled by distinct regulatory complexes utilizing specialized paralogs of TELOMERE REPEAT BINDING FACTORS
    Article Snippet: To check protein integrity, 1μg of protein was mixed 2x SDS-Loading buffer (126 mM Tris-HCl (pH 6.8), 20% glycerol, 4% SDS, 0.02% bromophenol blue), incubated at 95°C for 5min, and run in 1x TGS buffer (Bio Rad) on a 1.5mm, 12% SDS-PAGE at 100 V for approximately 1.5h. .. The SDS-PAGE was stained with Coomassie brilliant blue staining solution (1g Coomassie Brilliant Blue (Bio-Rad), 500ml MeOH, 100ml glacial acetic acid, 400ml H 2 O) and de-stained with H 2 O overnight. .. Forward and reverse 5’-Cy3-labelled oligonucleotides of 28 bp were ordered from SIGMA-ALDRICH.

    Staining:

    Article Title: Epigenetic gene regulation is controlled by distinct regulatory complexes utilizing specialized paralogs of TELOMERE REPEAT BINDING FACTORS
    Article Snippet: Protein quantity was determined by Bio-Rad Protein Assay according to manufacturer’s instructions. .. To check protein integrity, 1 μg of protein was mixed 2x SDS-Loading buffer (126 mM Tris-HCl (pH 6.8), 20 % glycerol, 4 % SDS, 0.02 % bromophenol blue), incubated at 95 °C for 5 min, and run in 1x TGS buffer (Bio Rad) on a 1.5 mm, 12 % SDS-PAGE at 100 V for approximately 1.5 h. The SDS-PAGE was stained with Coomassie brilliant blue staining solution (1 g Coomassie Brilliant Blue (Bio-Rad), 500 ml MeOH, 100 ml glacial acetic acid, 400 ml H 2 O) and de-stained with H 2 O overnight. .. Forward and reverse 5’-Cy3-labelled oligonucleotides of 28 bp were ordered from SIGMA-ALDRICH.

    Article Title: Proposal of Bacillus altaicus sp. nov. Isolated from Soil in the Altai Region, Russia.
    Article Snippet: .. Next, the culture was collected using a microbiological loop with a capacity of 10 μL and suspended in sterile deionized water on a slide, dried at room temperature, stained for 10–15 min with Coomassie Brilliant Blue Staining Solution (Bio-Rad Laboratories Inc., Hercules, CA, USA) using a Coplin jar, and rinsed with distilled water. .. The cells were examined under a light microscope (Carl Zeiss Axio Imager 2, Jena, Germany) at 1000× magnification.

    Article Title: Loss of SALL1 Promotes Hepatocellular Carcinoma Growth and Is Associated with Poor Clinical Outcome.
    Article Snippet: Band intensities were quantified using Image Lab software (Bio-Rad Laboratories, Hercules, CA, USA) and normalized to β-actin (ACTB) as an internal control. .. For total protein visualization, whole gels loaded with 30 μg of protein per lane were stained after SDS-PAGE with Coomassie Brilliant Blue staining solution (0.1% w/v, methanol: 45% v/v, acetic acid: 10%) (Bio-Rad Laboratories, Hercules, CA, USA; Cat. No. 161-0406). .. Gels were destained for https://doi.org/10.3390/cancers18091355 several hours in solution containing 45% methanol and 10% acetic acid (Sigma-Aldrich, St. Louis, MO, USA; Cat. No. 64-19-7), and protein levels in all lanes were assessed visually.

    Article Title: Investigation on physical and physiological properties of extracellular vesicles derived from Enterococcus faecalis.
    Article Snippet: .. SDS‐PAGE Equal amounts of each EV preparation were loaded onto a 15% acrylamide-containing gel and electrophoresed at 80–150 V for 2 h. After SDS-PAGE, the gel was stained with Coomassie brilliant blue staining solution (Bio-Rad), followed by destaining with methanol/acetic acid-containing solution. ..

    Article Title: Investigation on physical and physiological properties of extracellular vesicles derived from Enterococcus faecalis
    Article Snippet: .. Equal amounts of each EV preparation were loaded onto a 15% acrylamide-containing gel and electrophoresed at 80–150 V for 2 h. After SDS-PAGE, the gel was stained with Coomassie brilliant blue staining solution (Bio-Rad), followed by destaining with methanol/acetic acid-containing solution. ..

    Article Title: Proposal of Bacillus altaicus sp. nov. Isolated from Soil in the Altai Region, Russia
    Article Snippet: .. Next, the culture was collected using a microbiological loop with a capacity of 10 μL and suspended in sterile deionized water on a slide, dried at room temperature, stained for 10–15 min with Coomassie Brilliant Blue Staining Solution (Bio-Rad Laboratories Inc., Hercules, CA, USA) using a Coplin jar, and rinsed with distilled water. .. The cells were examined under a light microscope (Carl Zeiss Axio Imager 2, Jena, Germany) at 1000× magnification.

    Article Title: Correction to “Incorporation of Multiple β 2 ‑Hydroxy Acids into a Protein in Vivo Using an Orthogonal Aminoacyl-tRNA Synthetase”
    Article Snippet: .. 30 μL of sample was loaded per well to a Bio-Rad Any kDTM Mini-PROTEAN® TGXTM Precast Protein Gel and run at 200 V for 30 min. Gels were incubated in coomassie brilliant blue staining solution for 30 min followed by destaining solution for 30 min and imaged on a Bio-Rad ChemiDocTM MP Imaging System. ..

    Article Title: Epigenetic gene regulation is controlled by distinct regulatory complexes utilizing specialized paralogs of TELOMERE REPEAT BINDING FACTORS
    Article Snippet: To check protein integrity, 1μg of protein was mixed 2x SDS-Loading buffer (126 mM Tris-HCl (pH 6.8), 20% glycerol, 4% SDS, 0.02% bromophenol blue), incubated at 95°C for 5min, and run in 1x TGS buffer (Bio Rad) on a 1.5mm, 12% SDS-PAGE at 100 V for approximately 1.5h. .. The SDS-PAGE was stained with Coomassie brilliant blue staining solution (1g Coomassie Brilliant Blue (Bio-Rad), 500ml MeOH, 100ml glacial acetic acid, 400ml H 2 O) and de-stained with H 2 O overnight. .. Forward and reverse 5’-Cy3-labelled oligonucleotides of 28 bp were ordered from SIGMA-ALDRICH.

    Sterility:

    Article Title: Proposal of Bacillus altaicus sp. nov. Isolated from Soil in the Altai Region, Russia.
    Article Snippet: .. Next, the culture was collected using a microbiological loop with a capacity of 10 μL and suspended in sterile deionized water on a slide, dried at room temperature, stained for 10–15 min with Coomassie Brilliant Blue Staining Solution (Bio-Rad Laboratories Inc., Hercules, CA, USA) using a Coplin jar, and rinsed with distilled water. .. The cells were examined under a light microscope (Carl Zeiss Axio Imager 2, Jena, Germany) at 1000× magnification.

    Article Title: Proposal of Bacillus altaicus sp. nov. Isolated from Soil in the Altai Region, Russia
    Article Snippet: .. Next, the culture was collected using a microbiological loop with a capacity of 10 μL and suspended in sterile deionized water on a slide, dried at room temperature, stained for 10–15 min with Coomassie Brilliant Blue Staining Solution (Bio-Rad Laboratories Inc., Hercules, CA, USA) using a Coplin jar, and rinsed with distilled water. .. The cells were examined under a light microscope (Carl Zeiss Axio Imager 2, Jena, Germany) at 1000× magnification.

    Imaging:

    Article Title: Correction to “Incorporation of Multiple β 2 ‑Hydroxy Acids into a Protein in Vivo Using an Orthogonal Aminoacyl-tRNA Synthetase”
    Article Snippet: .. 30 μL of sample was loaded per well to a Bio-Rad Any kDTM Mini-PROTEAN® TGXTM Precast Protein Gel and run at 200 V for 30 min. Gels were incubated in coomassie brilliant blue staining solution for 30 min followed by destaining solution for 30 min and imaged on a Bio-Rad ChemiDocTM MP Imaging System. ..



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